TaqI
T▾CGA5′ overhang (2)
TaqI is a 4 bp-recognition restriction enzyme. It runs best in rCutSmart at 65°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 50% |
| rNEBuffer 2.1 | 75% |
| rNEBuffer 3.1 | 100% |
| rCutSmartbest | 100% |
- Incubation
- 65°C
- Heat inactivation
- 80°C, 20 min
- Star activity
- None
- Methylation blocked
- CpG
TCGA. Incubate at 65°C (thermophilic). Blocked by CpG (and overlapping Dam) methylation.
Find TaqI sites in your own sequence
Paste a sequence below and this scans it for every TaqI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does TaqI recognize?
TaqI recognizes the 4 bp sequence TCGA (5′→3′), cutting the top strand between position 1 and 2 within the site to leave a 5′ overhang of 2 bases.
What buffer should I use for TaqI?
rCutSmart gives the highest activity (incubate at 65°C). Chart entry 'TaqalphaI' (TaqI). rNEBuffer3.1 and rCutSmart both 100%.
Does TaqI have star activity?
No documented star activity under standard reaction conditions.
Is TaqI blocked by DNA methylation?
Yes — blocked by CpG methylation. Use methylation-free template (e.g. a dam⁻/dcm⁻ strain) if this matters for your digest.
Can I heat-inactivate TaqI?
Yes — 80°C for 20 minutes.