HaeIII
GG▾CCBlunt
HaeIII is a 4 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 50% |
| rNEBuffer 2.1 | 100% |
| rNEBuffer 3.1 | 25% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- 80°C, 20 min
- Star activity
- None
- Methylation blocked
- None
GGCC. NEB: not sensitive to Dam, Dcm or CpG methylation. (Product note: GGC(m5)C is cleaved, GG(m5)CC is resistant.)
Find HaeIII sites in your own sequence
Paste a sequence below and this scans it for every HaeIII recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Enzymes with ends compatible with HaeIII
HaeIII leaves blunt ends. Of the other 48 enzymes in the restriction enzyme table, these 10 leave an end that can be ligated to a HaeIII end:
Frequently asked questions
What does HaeIII recognize?
HaeIII recognizes the 4 bp sequence GGCC (5′→3′), cutting the top strand between position 2 and 3 within the site to leave a blunt.
What buffer should I use for HaeIII?
rCutSmart gives the highest activity (incubate at 37°C).
Does HaeIII have star activity?
No documented star activity under standard reaction conditions.
Is HaeIII blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate HaeIII?
Yes — 80°C for 20 minutes.