Restriction Enzyme Site Finder
Find recognition and cut sites for common restriction enzymes.
🔒 Runs entirely in your browser — nothing is uploaded
Find restriction enzyme recognition and cut sites in a DNA sequence using a curated set of common enzymes. IUPAC-aware matching handles degenerate recognition sequences, and you get cut positions and site counts — everything you need to plan a digest, design a cloning strategy or build a restriction map.
0 bp · 0 sites from 49 enzymes
Enter a sequence to scan.
How to use the Restriction Sites tool
- 1Paste the DNA sequence you want to digest or map.
- 2Select the enzymes to scan for, or use the common set.
- 3Read each enzyme's recognition sites, cut positions and total counts.
Frequently asked questions
- Which enzymes are included?
- A curated set of ~50 commonly used enzymes (EcoRI, BamHI, HindIII, NotI and many more), including some with degenerate recognition sequences.
- Are degenerate recognition sequences handled?
- Yes. Enzymes with IUPAC codes (e.g. HinfI G^ANTC) are matched correctly using the full ambiguity alphabet.