DraI
TTT▾AAABlunt
DraI is a 6 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 75% |
| rNEBuffer 2.1 | 75% |
| rNEBuffer 3.1 | 50% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- 65°C, 20 min
- Star activity
- None
- Methylation blocked
- None
TTTAAA. Supplied in rCutSmart (100%); the current product page also lists usable activity in r1.1/r2.1 (75%) and r3.1 (50%), unlike the older chart's N/R.
Find DraI sites in your own sequence
Paste a sequence below and this scans it for every DraI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Enzymes with ends compatible with DraI
DraI leaves blunt ends. Of the other 48 enzymes in the restriction enzyme table, these 10 leave an end that can be ligated to a DraI end:
Frequently asked questions
What does DraI recognize?
DraI recognizes the 6 bp sequence TTTAAA (5′→3′), cutting the top strand between position 3 and 4 within the site to leave a blunt.
What buffer should I use for DraI?
rCutSmart gives the highest activity (incubate at 37°C).
Does DraI have star activity?
No documented star activity under standard reaction conditions.
Is DraI blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate DraI?
Yes — 65°C for 20 minutes.