BspEI
T▾CCGGA5′ overhang (4)
BspEI is a 6 bp-recognition restriction enzyme. It runs best in rNEBuffer 3.1 at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 0% |
| rNEBuffer 2.1 | 10% |
| rNEBuffer 3.1best | 100% |
| rCutSmart | 0% |
- Incubation
- 37°C
- Heat inactivation
- 80°C, 20 min
- Star activity
- None
- Methylation blocked
- Dcm, CpG
TCCGGA. CpG blocks; Dcm overlap possible in context.
Find BspEI sites in your own sequence
Paste a sequence below and this scans it for every BspEI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does BspEI recognize?
BspEI recognizes the 6 bp sequence TCCGGA (5′→3′), cutting the top strand between position 1 and 2 within the site to leave a 5′ overhang of 4 bases.
What buffer should I use for BspEI?
rNEBuffer 3.1 gives the highest activity (incubate at 37°C).
Does BspEI have star activity?
No documented star activity under standard reaction conditions.
Is BspEI blocked by DNA methylation?
Yes — blocked by Dcm, CpG methylation. Use methylation-free template (e.g. a dam⁻/dcm⁻ strain) if this matters for your digest.
Can I heat-inactivate BspEI?
Yes — 80°C for 20 minutes.