BstBI
TT▾CGAA5′ overhang (2)
BstBI is a 6 bp-recognition restriction enzyme. It runs best in rCutSmart at 65°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 75% |
| rNEBuffer 2.1 | 50% |
| rNEBuffer 3.1 | 25% |
| rCutSmartbest | 100% |
- Incubation
- 65°C
- Heat inactivation
- Not inactivable
- Star activity
- None
- Methylation blocked
- Dam, CpG
TTCGAA. Incubate at 65°C (thermophilic). Not heat-inactivable. Dam-overlap and CpG block.
Find BstBI sites in your own sequence
Paste a sequence below and this scans it for every BstBI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does BstBI recognize?
BstBI recognizes the 6 bp sequence TTCGAA (5′→3′), cutting the top strand between position 2 and 3 within the site to leave a 5′ overhang of 2 bases.
What buffer should I use for BstBI?
rCutSmart gives the highest activity (incubate at 65°C).
Does BstBI have star activity?
No documented star activity under standard reaction conditions.
Is BstBI blocked by DNA methylation?
Yes — blocked by Dam, CpG methylation. Use methylation-free template (e.g. a dam⁻/dcm⁻ strain) if this matters for your digest.
Can I heat-inactivate BstBI?
No — BstBI is not heat-inactivable. Use a column cleanup or phenol-chloroform extraction to stop the reaction instead.