ScaI
AGT▾ACTBlunt
ScaI is a 6 bp-recognition restriction enzyme. It runs best in rNEBuffer 3.1 at 37°C, and is prone to star activity under non-standard conditions.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | N/R (not recommended) |
| rNEBuffer 2.1 | N/R (not recommended) |
| rNEBuffer 3.1best | 100% |
| rCutSmart | N/R (not recommended) |
- Incubation
- 37°C
- Heat inactivation
- 80°C, 20 min
- Star activity
- Yes
- Methylation blocked
- None
AGTACT. Chart lists N/R for 1.1/2.1/rCutSmart (only 100% in NEBuffer3.1). Values for ScaI (not ScaI-HF).
Find ScaI sites in your own sequence
Paste a sequence below and this scans it for every ScaI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does ScaI recognize?
ScaI recognizes the 6 bp sequence AGTACT (5′→3′), cutting the top strand between position 3 and 4 within the site to leave a blunt.
What buffer should I use for ScaI?
rNEBuffer 3.1 gives the highest activity (incubate at 37°C).
Does ScaI have star activity?
Yes — under non-standard conditions (excess enzyme/glycerol, long incubation, low ionic strength) it can cleave at sites resembling but not matching AGTACT.
Is ScaI blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate ScaI?
Yes — 80°C for 20 minutes.