ScaI
AGT▾ACTBlunt
ScaI is a 6 bp-recognition restriction enzyme. It runs best in rNEBuffer 3.1 at 37°C, and is prone to star activity under non-standard conditions.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | N/R (not recommended) |
| rNEBuffer 2.1 | N/R (not recommended) |
| rNEBuffer 3.1best | 100% |
| rCutSmart | N/R (not recommended) |
- Incubation
- 37°C
- Heat inactivation
- 80°C, 20 min
- Star activity
- Yes
- Methylation blocked
- None
AGTACT. Values are the classic non-HF ScaI (chart: N/R for 1.1/2.1/rCutSmart, 100% in NEBuffer 3.1) and are UNVERIFIED against a live NEB page — R0122 is discontinued and only ScaI-HF (R3122) remains, which differs (100/100/10/100, supplied in rCutSmart).
Find ScaI sites in your own sequence
Paste a sequence below and this scans it for every ScaI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Enzymes with ends compatible with ScaI
ScaI leaves blunt ends. Of the other 48 enzymes in the restriction enzyme table, these 10 leave an end that can be ligated to a ScaI end:
Frequently asked questions
What does ScaI recognize?
ScaI recognizes the 6 bp sequence AGTACT (5′→3′), cutting the top strand between position 3 and 4 within the site to leave a blunt.
What buffer should I use for ScaI?
rNEBuffer 3.1 gives the highest activity (incubate at 37°C).
Does ScaI have star activity?
Yes — under non-standard conditions (excess enzyme/glycerol, long incubation, low ionic strength) it can cleave at sites resembling but not matching AGTACT.
Is ScaI blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate ScaI?
Yes — 80°C for 20 minutes.