BstYI
R▾GATCY5′ overhang (4)
BstYI is a 6 bp-recognition restriction enzyme. It runs best in rCutSmart at 60°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 50% |
| rNEBuffer 2.1 | 100% |
| rNEBuffer 3.1 | 75% |
| rCutSmartbest | 100% |
- Incubation
- 60°C
- Heat inactivation
- 80°C, 20 min
- Star activity
- None
- Methylation blocked
- Dam
RGATCY (degenerate). Incubate at 60°C. Overlapping Dam (GATC core) methylation blocks.
Find BstYI sites in your own sequence
Paste a sequence below and this scans it for every BstYI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does BstYI recognize?
BstYI recognizes the 6 bp sequence RGATCY (5′→3′), cutting the top strand between position 1 and 2 within the site to leave a 5′ overhang of 4 bases.
What buffer should I use for BstYI?
rCutSmart gives the highest activity (incubate at 60°C). Supplied NEBuffer 2; rCutSmart 100% too.
Does BstYI have star activity?
No documented star activity under standard reaction conditions.
Is BstYI blocked by DNA methylation?
Yes — blocked by Dam methylation. Use methylation-free template (e.g. a dam⁻/dcm⁻ strain) if this matters for your digest.
Can I heat-inactivate BstYI?
Yes — 80°C for 20 minutes.