MluI
A▾CGCGT5′ overhang (4)
MluI is a 6 bp-recognition restriction enzyme. It runs best in rNEBuffer 3.1 at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 25% |
| rNEBuffer 2.1 | 75% |
| rNEBuffer 3.1best | 100% |
| rCutSmart | 50% |
- Incubation
- 37°C
- Heat inactivation
- 65°C, 20 min
- Star activity
- None
- Methylation blocked
- CpG
ACGCGT contains CG; CpG methylation blocks.
Find MluI sites in your own sequence
Paste a sequence below and this scans it for every MluI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does MluI recognize?
MluI recognizes the 6 bp sequence ACGCGT (5′→3′), cutting the top strand between position 1 and 2 within the site to leave a 5′ overhang of 4 bases.
What buffer should I use for MluI?
rNEBuffer 3.1 gives the highest activity (incubate at 37°C).
Does MluI have star activity?
No documented star activity under standard reaction conditions.
Is MluI blocked by DNA methylation?
Yes — blocked by CpG methylation. Use methylation-free template (e.g. a dam⁻/dcm⁻ strain) if this matters for your digest.
Can I heat-inactivate MluI?
Yes — 65°C for 20 minutes.