MspI
C▾CGG5′ overhang (2)
MspI is a 4 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 75% |
| rNEBuffer 2.1 | 100% |
| rNEBuffer 3.1 | 50% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- Not inactivable
- Star activity
- None
- Methylation blocked
- None
CCGG. Notably CpG-METHYLATION TOLERANT (cuts C(5me)CGG) — its isoschizomer HpaII is blocked. Not heat-inactivable.
Find MspI sites in your own sequence
Paste a sequence below and this scans it for every MspI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does MspI recognize?
MspI recognizes the 4 bp sequence CCGG (5′→3′), cutting the top strand between position 1 and 2 within the site to leave a 5′ overhang of 2 bases.
What buffer should I use for MspI?
rCutSmart gives the highest activity (incubate at 37°C).
Does MspI have star activity?
No documented star activity under standard reaction conditions.
Is MspI blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate MspI?
No — MspI is not heat-inactivable. Use a column cleanup or phenol-chloroform extraction to stop the reaction instead.