PmeI
GTTT▾AAACBlunt
PmeI is a 8 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 0% |
| rNEBuffer 2.1 | 50% |
| rNEBuffer 3.1 | 10% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- 65°C, 20 min
- Star activity
- None
- Methylation blocked
- None
GTTTAAAC.
Find PmeI sites in your own sequence
Paste a sequence below and this scans it for every PmeI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does PmeI recognize?
PmeI recognizes the 8 bp sequence GTTTAAAC (5′→3′), cutting the top strand between position 4 and 5 within the site to leave a blunt.
What buffer should I use for PmeI?
rCutSmart gives the highest activity (incubate at 37°C). Supplied NEBuffer 4 + BSA == rCutSmart.
Does PmeI have star activity?
No documented star activity under standard reaction conditions.
Is PmeI blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate PmeI?
Yes — 65°C for 20 minutes.