PmeI
GTTT▾AAACBlunt
PmeI is a 8 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 0% |
| rNEBuffer 2.1 | 50% |
| rNEBuffer 3.1 | 10% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- 65°C, 20 min
- Star activity
- None
- Methylation blocked
- CpG
GTTTAAAC. NEB reports CpG blocking for some combinations of overlapping methylation.
Find PmeI sites in your own sequence
Paste a sequence below and this scans it for every PmeI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Enzymes with ends compatible with PmeI
PmeI leaves blunt ends. Of the other 48 enzymes in the restriction enzyme table, these 10 leave an end that can be ligated to a PmeI end:
Frequently asked questions
What does PmeI recognize?
PmeI recognizes the 8 bp sequence GTTTAAAC (5′→3′), cutting the top strand between position 4 and 5 within the site to leave a blunt.
What buffer should I use for PmeI?
rCutSmart gives the highest activity (incubate at 37°C). Supplied NEBuffer 4 + BSA == rCutSmart.
Does PmeI have star activity?
No documented star activity under standard reaction conditions.
Is PmeI blocked by DNA methylation?
Yes — blocked by CpG methylation. Use methylation-free template (e.g. a dam⁻/dcm⁻ strain) if this matters for your digest.
Can I heat-inactivate PmeI?
Yes — 65°C for 20 minutes.