PvuII
CAG▾CTGBlunt
PvuII is a 6 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C, and is prone to star activity under non-standard conditions.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 50% |
| rNEBuffer 2.1 | 100% |
| rNEBuffer 3.1 | 100% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- Not inactivable
- Star activity
- High propensity
- Methylation blocked
- None
CAGCTG. Not heat-inactivable. Documented star activity (PvuII*).
Find PvuII sites in your own sequence
Paste a sequence below and this scans it for every PvuII recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Enzymes with ends compatible with PvuII
PvuII leaves blunt ends. Of the other 48 enzymes in the restriction enzyme table, these 10 leave an end that can be ligated to a PvuII end:
Frequently asked questions
What does PvuII recognize?
PvuII recognizes the 6 bp sequence CAGCTG (5′→3′), cutting the top strand between position 3 and 4 within the site to leave a blunt.
What buffer should I use for PvuII?
rCutSmart gives the highest activity (incubate at 37°C). Supplied in NEBuffer r3.1; 100% there and in r2.1/rCutSmart (r1.1 is 50%).
Does PvuII have star activity?
Yes, and it has a notably high propensity for it — under non-standard conditions (excess enzyme/glycerol, long incubation, low ionic strength) it can cleave at sites resembling but not matching CAGCTG.
Is PvuII blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate PvuII?
No — PvuII is not heat-inactivable. Use a column cleanup or phenol-chloroform extraction to stop the reaction instead.