AgeI
A▾CCGGT5′ overhang (4)
AgeI is a 6 bp-recognition restriction enzyme. It runs best in rNEBuffer 1.1 at 37°C, and is prone to star activity under non-standard conditions.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1best | 100% |
| rNEBuffer 2.1 | 50% |
| rNEBuffer 3.1 | 10% |
| rCutSmart | 75% |
- Incubation
- 37°C
- Heat inactivation
- 65°C, 20 min
- Star activity
- Yes
- Methylation blocked
- CpG
ACCGGT contains CG; CpG blocks. AgeI (not HF) has star propensity; consider AgeI-HF.
Find AgeI sites in your own sequence
Paste a sequence below and this scans it for every AgeI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does AgeI recognize?
AgeI recognizes the 6 bp sequence ACCGGT (5′→3′), cutting the top strand between position 1 and 2 within the site to leave a 5′ overhang of 4 bases.
What buffer should I use for AgeI?
rNEBuffer 1.1 gives the highest activity (incubate at 37°C).
Does AgeI have star activity?
Yes — under non-standard conditions (excess enzyme/glycerol, long incubation, low ionic strength) it can cleave at sites resembling but not matching ACCGGT.
Is AgeI blocked by DNA methylation?
Yes — blocked by CpG methylation. Use methylation-free template (e.g. a dam⁻/dcm⁻ strain) if this matters for your digest.
Can I heat-inactivate AgeI?
Yes — 65°C for 20 minutes.