SeqBench

Free Online Bioinformatics Tools

Fast, private bioinformatics tools — right in your browser.

12 DNA, RNA and protein tools. No sign-up, no uploads — every calculation happens locally in your browser.

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Sequence manipulation

Reverse Complement

Get the reverse, complement or reverse complement of a DNA/RNA sequence.

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Sequence Formatter

Clean, case-convert, wrap, reverse and convert between DNA and RNA.

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Random Sequence

Generate random sequences with an optional GC target.

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Translation & ORFs

Translate DNA

Translate a nucleotide sequence to protein in any or all six reading frames.

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ORF Finder

Find open reading frames in all six frames and translate them.

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Codon Optimizer

Optimise a protein or CDS for expression in E. coli, human or yeast.

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Analysis & design

GC Content

Calculate GC%, AT% and per-base composition of a sequence.

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Primer Tm

Estimate primer Tm, GC% and molecular weight from a sequence.

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Restriction Sites

Find recognition and cut sites for common restriction enzymes.

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FASTA/FASTQ Stats

Summarise and validate FASTA or FASTQ: counts, N50, GC, quality.

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Alignment & visualization

Pairwise Alignment

Align two sequences with Needleman-Wunsch or Smith-Waterman.

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Plasmid Viewer

Render a circular or linear map with restriction sites.

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Bioinformatics tools that run in your browser

SeqBench is a free collection of fast, privacy-friendly bioinformatics tools for students, researchers and anyone working with DNA, RNA and protein sequences. Calculate the reverse complement of a sequence, translate DNA to protein, find open reading frames, check GC content and primer melting temperature, scan for restriction sites, and more — all without installing software or creating an account. Because every tool runs entirely in your browser, your sequences never leave your computer.

Frequently asked questions

Are these bioinformatics tools really free?
Yes. Every tool on SeqBench is free to use with no sign-up, no account and no usage limits.
Is my sequence data private?
Completely. Every calculation runs in your browser with JavaScript. Your DNA, RNA or protein sequences are never uploaded, logged or stored — which makes the tools safe for unpublished or sensitive data.
Do I need to install anything?
No. SeqBench runs in any modern web browser on desktop or mobile. There is nothing to download or configure.
What can I do with SeqBench?
Compute reverse complements, translate DNA to protein, find ORFs, calculate GC content and primer Tm, scan for restriction sites, optimise codons, align two sequences, view plasmid maps, summarise FASTA/FASTQ files and more.