HpaI
GTT▾AACBlunt
HpaI is a 6 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C, and is prone to star activity under non-standard conditions.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 25% |
| rNEBuffer 2.1 | 25% |
| rNEBuffer 3.1 | 10% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- Not inactivable
- Star activity
- Yes
- Methylation blocked
- None
GTTAAC. Not heat-inactivable. Some star propensity.
Find HpaI sites in your own sequence
Paste a sequence below and this scans it for every HpaI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does HpaI recognize?
HpaI recognizes the 6 bp sequence GTTAAC (5′→3′), cutting the top strand between position 3 and 4 within the site to leave a blunt.
What buffer should I use for HpaI?
rCutSmart gives the highest activity (incubate at 37°C).
Does HpaI have star activity?
Yes — under non-standard conditions (excess enzyme/glycerol, long incubation, low ionic strength) it can cleave at sites resembling but not matching GTTAAC.
Is HpaI blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate HpaI?
No — HpaI is not heat-inactivable. Use a column cleanup or phenol-chloroform extraction to stop the reaction instead.