SbfI
CCTGCA▾GG3′ overhang (4)
SbfI is a 8 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C, and is prone to star activity under non-standard conditions.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 50% |
| rNEBuffer 2.1 | 25% |
| rNEBuffer 3.1 | 0% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- 80°C, 20 min
- Star activity
- Yes
- Methylation blocked
- None
CCTGCAGG. NEB reports no Dam/Dcm/CpG sensitivity. Star activity may result from a glycerol concentration of >5%.
Find SbfI sites in your own sequence
Paste a sequence below and this scans it for every SbfI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does SbfI recognize?
SbfI recognizes the 8 bp sequence CCTGCAGG (5′→3′), cutting the top strand between position 6 and 7 within the site to leave a 3′ overhang of 4 bases.
What buffer should I use for SbfI?
rCutSmart gives the highest activity (incubate at 37°C).
Does SbfI have star activity?
Yes — under non-standard conditions (excess enzyme/glycerol, long incubation, low ionic strength) it can cleave at sites resembling but not matching CCTGCAGG.
Is SbfI blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate SbfI?
Yes — 80°C for 20 minutes.