SwaI
ATTT▾AAATBlunt
SwaI is a 8 bp-recognition restriction enzyme. It runs best in rNEBuffer 3.1 at 25°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 10% |
| rNEBuffer 2.1 | 10% |
| rNEBuffer 3.1best | 100% |
| rCutSmart | 10% |
- Incubation
- 25°C
- Heat inactivation
- 65°C, 20 min
- Star activity
- None
- Methylation blocked
- None
ATTTAAAT. Incubate at 25°C.
Find SwaI sites in your own sequence
Paste a sequence below and this scans it for every SwaI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Enzymes with ends compatible with SwaI
SwaI leaves blunt ends. Of the other 48 enzymes in the restriction enzyme table, these 10 leave an end that can be ligated to a SwaI end:
Frequently asked questions
What does SwaI recognize?
SwaI recognizes the 8 bp sequence ATTTAAAT (5′→3′), cutting the top strand between position 4 and 5 within the site to leave a blunt.
What buffer should I use for SwaI?
rNEBuffer 3.1 gives the highest activity (incubate at 25°C).
Does SwaI have star activity?
No documented star activity under standard reaction conditions.
Is SwaI blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate SwaI?
Yes — 65°C for 20 minutes.