SwaI
ATTT▾AAATBlunt
SwaI is a 8 bp-recognition restriction enzyme. It runs best in rNEBuffer 3.1 at 25°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 10% |
| rNEBuffer 2.1 | 10% |
| rNEBuffer 3.1best | 100% |
| rCutSmart | 10% |
- Incubation
- 25°C
- Heat inactivation
- 65°C, 20 min
- Star activity
- None
- Methylation blocked
- None
ATTTAAAT. Incubate at 25°C.
Find SwaI sites in your own sequence
Paste a sequence below and this scans it for every SwaI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does SwaI recognize?
SwaI recognizes the 8 bp sequence ATTTAAAT (5′→3′), cutting the top strand between position 4 and 5 within the site to leave a blunt.
What buffer should I use for SwaI?
rNEBuffer 3.1 gives the highest activity (incubate at 25°C).
Does SwaI have star activity?
No documented star activity under standard reaction conditions.
Is SwaI blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate SwaI?
Yes — 65°C for 20 minutes.