HinfI
G▾ANTC5′ overhang (3)
HinfI is a 5 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 75% |
| rNEBuffer 2.1 | 100% |
| rNEBuffer 3.1 | 75% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- 80°C, 20 min
- Star activity
- None
- Methylation blocked
- None
GANTC (degenerate).
Find HinfI sites in your own sequence
Paste a sequence below and this scans it for every HinfI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does HinfI recognize?
HinfI recognizes the 5 bp sequence GANTC (5′→3′), cutting the top strand between position 1 and 2 within the site to leave a 5′ overhang of 3 bases.
What buffer should I use for HinfI?
rCutSmart gives the highest activity (incubate at 37°C).
Does HinfI have star activity?
No documented star activity under standard reaction conditions.
Is HinfI blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate HinfI?
Yes — 80°C for 20 minutes.