HinfI
G▾ANTC5′ overhang (3)
HinfI is a 5 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 50% |
| rNEBuffer 2.1 | 100% |
| rNEBuffer 3.1 | 100% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- 80°C, 20 min
- Star activity
- None
- Methylation blocked
- CpG
GANTC (degenerate). NEB reports CpG blocking for some combinations of overlapping methylation.
Find HinfI sites in your own sequence
Paste a sequence below and this scans it for every HinfI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Enzymes with ends compatible with HinfI
HinfI's overhang falls on a degenerate position of GANTC (ANT), so which bases protrude depends on the template and compatibility cannot be settled from the enzyme alone.
Frequently asked questions
What does HinfI recognize?
HinfI recognizes the 5 bp sequence GANTC (5′→3′), cutting the top strand between position 1 and 2 within the site to leave a 5′ overhang of 3 bases.
What buffer should I use for HinfI?
rCutSmart gives the highest activity (incubate at 37°C).
Does HinfI have star activity?
No documented star activity under standard reaction conditions.
Is HinfI blocked by DNA methylation?
Yes — blocked by CpG methylation. Use methylation-free template (e.g. a dam⁻/dcm⁻ strain) if this matters for your digest.
Can I heat-inactivate HinfI?
Yes — 80°C for 20 minutes.