AflII
C▾TTAAG5′ overhang (4)
AflII is a 6 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 50% |
| rNEBuffer 2.1 | 100% |
| rNEBuffer 3.1 | 10% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- 65°C, 20 min
- Star activity
- None
- Methylation blocked
- None
CTTAAG.
Find AflII sites in your own sequence
Paste a sequence below and this scans it for every AflII recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Enzymes with ends compatible with AflII
AflII leaves a 4 nt 5′ TTAA overhang. None of the other 48 enzymes in the restriction enzyme table leaves an end that can be ligated to an AflII end.
Frequently asked questions
What does AflII recognize?
AflII recognizes the 6 bp sequence CTTAAG (5′→3′), cutting the top strand between position 1 and 2 within the site to leave a 5′ overhang of 4 bases.
What buffer should I use for AflII?
rCutSmart gives the highest activity (incubate at 37°C). Supplied NEBuffer 4 + BSA == rCutSmart.
Does AflII have star activity?
No documented star activity under standard reaction conditions.
Is AflII blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate AflII?
Yes — 65°C for 20 minutes.