AluI
AG▾CTBlunt
AluI is a 4 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 25% |
| rNEBuffer 2.1 | 100% |
| rNEBuffer 3.1 | 50% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- 80°C, 20 min
- Star activity
- None
- Methylation blocked
- None
AGCT. NEB reports no Dam/Dcm/CpG sensitivity.
Find AluI sites in your own sequence
Paste a sequence below and this scans it for every AluI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Enzymes with ends compatible with AluI
AluI leaves blunt ends. Of the other 48 enzymes in the restriction enzyme table, these 10 leave an end that can be ligated to an AluI end:
Frequently asked questions
What does AluI recognize?
AluI recognizes the 4 bp sequence AGCT (5′→3′), cutting the top strand between position 2 and 3 within the site to leave a blunt.
What buffer should I use for AluI?
rCutSmart gives the highest activity (incubate at 37°C).
Does AluI have star activity?
No documented star activity under standard reaction conditions.
Is AluI blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate AluI?
Yes — 80°C for 20 minutes.