AluI
AG▾CTBlunt
AluI is a 4 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 25% |
| rNEBuffer 2.1 | 100% |
| rNEBuffer 3.1 | 50% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- 80°C, 20 min
- Star activity
- None
- Methylation blocked
- None
AGCT. NEB reports no Dam/Dcm/CpG sensitivity.
Find AluI sites in your own sequence
Paste a sequence below and this scans it for every AluI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does AluI recognize?
AluI recognizes the 4 bp sequence AGCT (5′→3′), cutting the top strand between position 2 and 3 within the site to leave a blunt.
What buffer should I use for AluI?
rCutSmart gives the highest activity (incubate at 37°C).
Does AluI have star activity?
No documented star activity under standard reaction conditions.
Is AluI blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate AluI?
Yes — 80°C for 20 minutes.