Free Online Bioinformatics Tools

Paste a sequence, get composition, ORFs, restriction sites and primers at once.

Get the reverse, complement or reverse complement of a DNA/RNA sequence.

Clean, case-convert, wrap, reverse and convert between DNA and RNA.

Generate random sequences with an optional GC target.

Search a sequence for a motif with IUPAC codes, mismatches and both strands.

Translate a nucleotide sequence to protein in any or all six reading frames.

Find open reading frames in all six frames and translate them.

Optimise a protein or CDS for expression in E. coli, human or yeast.

Calculate GC%, AT% and per-base composition of a sequence.

Estimate primer Tm, GC% and molecular weight from a sequence.

Find recognition and cut sites for common restriction enzymes.

Summarise and validate FASTA or FASTQ: counts, N50, GC, quality.

Convert between mass, moles, molarity and copy number for DNA/RNA.

Compute molecular weight, isoelectric point, extinction coefficient and composition.

Align two sequences with Needleman-Wunsch or Smith-Waterman.

Render a circular or linear map with restriction sites.