BglII
A▾GATCT5′ overhang (4)
BglII is a 6 bp-recognition restriction enzyme. It runs best in rNEBuffer 3.1 at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 10% |
| rNEBuffer 2.1 | 75% |
| rNEBuffer 3.1best | 100% |
| rCutSmart | 10% |
- Incubation
- 37°C
- Heat inactivation
- Not inactivable
- Star activity
- None
- Methylation blocked
- None
AGATCT. Not heat-inactivable.
Find BglII sites in your own sequence
Paste a sequence below and this scans it for every BglII recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does BglII recognize?
BglII recognizes the 6 bp sequence AGATCT (5′→3′), cutting the top strand between position 1 and 2 within the site to leave a 5′ overhang of 4 bases.
What buffer should I use for BglII?
rNEBuffer 3.1 gives the highest activity (incubate at 37°C).
Does BglII have star activity?
No documented star activity under standard reaction conditions.
Is BglII blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate BglII?
No — BglII is not heat-inactivable. Use a column cleanup or phenol-chloroform extraction to stop the reaction instead.