MboI
▾GATC5′ overhang (4)
MboI is a 4 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 75% |
| rNEBuffer 2.1 | 100% |
| rNEBuffer 3.1 | 100% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- 65°C, 20 min
- Star activity
- None
- Methylation blocked
- Dam
GATC. Blocked by Dam methylation (GA(me)TC) — do NOT use on Dam+ E. coli DNA. Isoschizomer DpnII also Dam-blocked; DpnI requires Dam methylation.
Find MboI sites in your own sequence
Paste a sequence below and this scans it for every MboI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does MboI recognize?
MboI recognizes the 4 bp sequence GATC (5′→3′), cutting the top strand between position 0 and 1 within the site to leave a 5′ overhang of 4 bases.
What buffer should I use for MboI?
rCutSmart gives the highest activity (incubate at 37°C).
Does MboI have star activity?
No documented star activity under standard reaction conditions.
Is MboI blocked by DNA methylation?
Yes — blocked by Dam methylation. Use methylation-free template (e.g. a dam⁻/dcm⁻ strain) if this matters for your digest.
Can I heat-inactivate MboI?
Yes — 65°C for 20 minutes.