DdeI
C▾TNAG5′ overhang (3)
DdeI is a 5 bp-recognition restriction enzyme. It runs best in rNEBuffer 3.1 at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 75% |
| rNEBuffer 2.1 | 100% |
| rNEBuffer 3.1best | 100% |
| rCutSmart | 75% |
- Incubation
- 37°C
- Heat inactivation
- 65°C, 20 min
- Star activity
- None
- Methylation blocked
- None
CTNAG (degenerate).
Find DdeI sites in your own sequence
Paste a sequence below and this scans it for every DdeI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does DdeI recognize?
DdeI recognizes the 5 bp sequence CTNAG (5′→3′), cutting the top strand between position 1 and 2 within the site to leave a 5′ overhang of 3 bases.
What buffer should I use for DdeI?
rNEBuffer 3.1 gives the highest activity (incubate at 37°C). rNEBuffer2.1 and 3.1 both 100%; chart supplies NEBuffer 3.
Does DdeI have star activity?
No documented star activity under standard reaction conditions.
Is DdeI blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate DdeI?
Yes — 65°C for 20 minutes.