DdeI
C▾TNAG5′ overhang (3)
DdeI is a 5 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 75% |
| rNEBuffer 2.1 | 100% |
| rNEBuffer 3.1 | 100% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- 65°C, 20 min
- Star activity
- None
- Methylation blocked
- None
CTNAG (degenerate).
Find DdeI sites in your own sequence
Paste a sequence below and this scans it for every DdeI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Enzymes with ends compatible with DdeI
DdeI's overhang falls on a degenerate position of CTNAG (TNA), so which bases protrude depends on the template and compatibility cannot be settled from the enzyme alone.
Frequently asked questions
What does DdeI recognize?
DdeI recognizes the 5 bp sequence CTNAG (5′→3′), cutting the top strand between position 1 and 2 within the site to leave a 5′ overhang of 3 bases.
What buffer should I use for DdeI?
rCutSmart gives the highest activity (incubate at 37°C). Supplied in rCutSmart; r2.1, r3.1 and rCutSmart are all 100% (r1.1 75%).
Does DdeI have star activity?
No documented star activity under standard reaction conditions.
Is DdeI blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate DdeI?
Yes — 65°C for 20 minutes.