DdeI
C▾TNAG5′ overhang (3)
DdeI is a 5 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 75% |
| rNEBuffer 2.1 | 100% |
| rNEBuffer 3.1 | 100% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- 65°C, 20 min
- Star activity
- None
- Methylation blocked
- None
CTNAG (degenerate).
Find DdeI sites in your own sequence
Paste a sequence below and this scans it for every DdeI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does DdeI recognize?
DdeI recognizes the 5 bp sequence CTNAG (5′→3′), cutting the top strand between position 1 and 2 within the site to leave a 5′ overhang of 3 bases.
What buffer should I use for DdeI?
rCutSmart gives the highest activity (incubate at 37°C). Supplied in rCutSmart; r2.1, r3.1 and rCutSmart are all 100% (r1.1 75%).
Does DdeI have star activity?
No documented star activity under standard reaction conditions.
Is DdeI blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate DdeI?
Yes — 65°C for 20 minutes.