StuI
AGG▾CCTBlunt
StuI is a 6 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 50% |
| rNEBuffer 2.1 | 100% |
| rNEBuffer 3.1 | 50% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- Not inactivable
- Star activity
- None
- Methylation blocked
- Dcm
AGGCCT. Not heat-inactivable. Dcm (CCWGG on complement) can overlap -> blocked in context.
Find StuI sites in your own sequence
Paste a sequence below and this scans it for every StuI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Enzymes with ends compatible with StuI
StuI leaves blunt ends. Of the other 48 enzymes in the restriction enzyme table, these 10 leave an end that can be ligated to a StuI end:
Frequently asked questions
What does StuI recognize?
StuI recognizes the 6 bp sequence AGGCCT (5′→3′), cutting the top strand between position 3 and 4 within the site to leave a blunt.
What buffer should I use for StuI?
rCutSmart gives the highest activity (incubate at 37°C).
Does StuI have star activity?
No documented star activity under standard reaction conditions.
Is StuI blocked by DNA methylation?
Yes — blocked by Dcm methylation. Use methylation-free template (e.g. a dam⁻/dcm⁻ strain) if this matters for your digest.
Can I heat-inactivate StuI?
No — StuI is not heat-inactivable. Use a column cleanup or phenol-chloroform extraction to stop the reaction instead.