EcoRV
GAT▾ATCBlunt
EcoRV is a 6 bp-recognition restriction enzyme. It runs best in rNEBuffer 3.1 at 37°C, and is prone to star activity under non-standard conditions.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 10% |
| rNEBuffer 2.1 | 50% |
| rNEBuffer 3.1best | 100% |
| rCutSmart | 10% |
- Incubation
- 37°C
- Heat inactivation
- 80°C, 20 min
- Star activity
- High propensity
- Methylation blocked
- CpG
GATATC. NEB reports CpG *impairs* (does not fully block) cleavage for some overlapping combinations; Dam/Dcm not sensitive. Documented star activity.
Find EcoRV sites in your own sequence
Paste a sequence below and this scans it for every EcoRV recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Enzymes with ends compatible with EcoRV
EcoRV leaves blunt ends. Of the other 48 enzymes in the restriction enzyme table, these 10 leave an end that can be ligated to an EcoRV end:
Frequently asked questions
What does EcoRV recognize?
EcoRV recognizes the 6 bp sequence GATATC (5′→3′), cutting the top strand between position 3 and 4 within the site to leave a blunt.
What buffer should I use for EcoRV?
rNEBuffer 3.1 gives the highest activity (incubate at 37°C).
Does EcoRV have star activity?
Yes, and it has a notably high propensity for it — under non-standard conditions (excess enzyme/glycerol, long incubation, low ionic strength) it can cleave at sites resembling but not matching GATATC.
Is EcoRV blocked by DNA methylation?
Yes — blocked by CpG methylation. Use methylation-free template (e.g. a dam⁻/dcm⁻ strain) if this matters for your digest.
Can I heat-inactivate EcoRV?
Yes — 80°C for 20 minutes.