ScrFI
CC▾NGG5′ overhang (1)
ScrFI is a 5 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 100% |
| rNEBuffer 2.1 | 100% |
| rNEBuffer 3.1 | 100% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- 65°C, 20 min
- Star activity
- None
- Methylation blocked
- Dcm, CpG
CCNGG. Dcm (CCWGG) overlaps directly -> blocked. CpG-context can block. 100% in all four buffers.
Find ScrFI sites in your own sequence
Paste a sequence below and this scans it for every ScrFI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does ScrFI recognize?
ScrFI recognizes the 5 bp sequence CCNGG (5′→3′), cutting the top strand between position 2 and 3 within the site to leave a 5′ overhang of 1 base.
What buffer should I use for ScrFI?
rCutSmart gives the highest activity (incubate at 37°C).
Does ScrFI have star activity?
No documented star activity under standard reaction conditions.
Is ScrFI blocked by DNA methylation?
Yes — blocked by Dcm, CpG methylation. Use methylation-free template (e.g. a dam⁻/dcm⁻ strain) if this matters for your digest.
Can I heat-inactivate ScrFI?
Yes — 65°C for 20 minutes.