PacI
TTAAT▾TAA3′ overhang (2)
PacI is a 8 bp-recognition restriction enzyme. It runs best in rCutSmart at 37°C.
NEB buffer activity
| Buffer | % Activity |
|---|---|
| rNEBuffer 1.1 | 100% |
| rNEBuffer 2.1 | 75% |
| rNEBuffer 3.1 | 10% |
| rCutSmartbest | 100% |
- Incubation
- 37°C
- Heat inactivation
- 65°C, 20 min
- Star activity
- None
- Methylation blocked
- None
TTAATTAA.
Find PacI sites in your own sequence
Paste a sequence below and this scans it for every PacI recognition site — the same engine behind the full Restriction Sites tool, scoped to just this one enzyme.
Frequently asked questions
What does PacI recognize?
PacI recognizes the 8 bp sequence TTAATTAA (5′→3′), cutting the top strand between position 5 and 6 within the site to leave a 3′ overhang of 2 bases.
What buffer should I use for PacI?
rCutSmart gives the highest activity (incubate at 37°C). Supplied NEBuffer 1 + BSA; rCutSmart 100% too -> prefer rCutSmart.
Does PacI have star activity?
No documented star activity under standard reaction conditions.
Is PacI blocked by DNA methylation?
Not blocked by Dam, Dcm, or CpG methylation.
Can I heat-inactivate PacI?
Yes — 65°C for 20 minutes.