Translate DNA to Protein (Six-Frame)
Translate a nucleotide sequence to protein in any or all six reading frames.
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Translate a DNA or RNA sequence into protein using the standard genetic code. Translate a single reading frame or all six frames at once, and switch between one- and three-letter amino-acid codes. Useful for confirming a coding sequence, finding the correct frame, or checking for premature stop codons.
0 bases
Enter a sequence to translate.
Working on a whole construct rather than one sequence? Open SeqStudio — the full editor, with live feature annotation, plasmid maps, restriction and primer panels, undo/redo, multi-document tabs and GenBank / SnapGene import and export.
How to use the Translate DNA tool
- 1Paste your nucleotide sequence (FASTA is accepted).
- 2Pick a single reading frame or choose six-frame translation.
- 3Select one- or three-letter amino-acid output and read the protein; stop codons appear as '*'.
Frequently asked questions
Which genetic code is used?
The Standard Genetic Code (NCBI translation table 1). Stop codons are shown as '*'.
What does six-frame translation mean?
There are three reading frames on the forward strand and three on the reverse strand. Six-frame translation shows the protein for all of them so you can spot the correct ORF.
What does 'X' mean in the output?
X marks a codon that cannot be resolved to a single amino acid. A codon containing an ambiguity code is only X when the ambiguity actually changes the residue — CTN is translated as Leu because every codon it covers is Leu, while NNN or TGN stay X.