Translate DNA to Protein (Six-Frame)
Translate a nucleotide sequence to protein in all six reading frames at once.
Translate a DNA or RNA sequence into protein using the standard genetic code. All six reading frames are shown together — there is nothing to choose — and each one reports its longest stretch carrying no stop codon, with the winner marked, so the coding frame is the one you can see rather than the one you have to hunt for. Useful for confirming a coding sequence, finding the correct frame, or checking for premature stop codons.
0 bases
Paste a nucleotide sequence above to translate it in all six reading frames.
Design, edit, and verify complete constructsSeqStudio combines sequence editing and annotation, plasmid maps, primer/cloning/CRISPR design, Sanger verification, and GenBank/SnapGene files in one workspace.
How to use the Translate DNA tool
- 1Paste your nucleotide sequence (FASTA is accepted).
- 2Read all six frames at once; stop codons appear as '*'.
- 3Check the “longest open reading” badge and the per-frame “aa open” figure to find the coding frame, then copy it.
Frequently asked questions
Which genetic code is used?
The Standard Genetic Code (NCBI translation table 1). Stop codons are shown as '*'.
What does six-frame translation mean?
There are three reading frames on the forward strand and three on the reverse strand. Six-frame translation shows the protein for all of them so you can spot the correct ORF.
What does 'X' mean in the output?
X marks a codon that cannot be resolved to a single amino acid. A codon containing an ambiguity code is only X when the ambiguity actually changes the residue — CTN is translated as Leu because every codon it covers is Leu, while NNN or TGN stay X.