SeqBench

Translate DNA to Protein (Six-Frame)

Translate a nucleotide sequence to protein in any or all six reading frames.

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Translate a DNA or RNA sequence into protein using the standard genetic code. Translate a single reading frame or all six frames at once, and switch between one- and three-letter amino-acid codes. Useful for confirming a coding sequence, finding the correct frame, or checking for premature stop codons.

0 bases

Frame +1 (+ strand)
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Frame +2 (+ strand)
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Frame +3 (+ strand)
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Frame -1 (- strand)
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Frame -2 (- strand)
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Frame -3 (- strand)
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How to use the Translate DNA tool

  1. 1Paste your nucleotide sequence (FASTA is accepted).
  2. 2Pick a single reading frame or choose six-frame translation.
  3. 3Select one- or three-letter amino-acid output and read the protein; stop codons appear as '*'.

Frequently asked questions

Which genetic code is used?
The Standard Genetic Code (NCBI translation table 1). Stop codons are shown as '*'.
What does six-frame translation mean?
There are three reading frames on the forward strand and three on the reverse strand. Six-frame translation shows the protein for all of them so you can spot the correct ORF.
What does 'X' mean in the output?
X marks a codon that contains an ambiguous base (e.g. N) and therefore cannot be unambiguously translated.

Learn more

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