Translate DNA to Protein (Six-Frame)
Translate a nucleotide sequence to protein in any or all six reading frames.
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Translate a DNA or RNA sequence into protein using the standard genetic code. Translate a single reading frame or all six frames at once, and switch between one- and three-letter amino-acid codes. Useful for confirming a coding sequence, finding the correct frame, or checking for premature stop codons.
0 bases
Frame +1 (+ strand)
βFrame +2 (+ strand)
βFrame +3 (+ strand)
βFrame -1 (- strand)
βFrame -2 (- strand)
βFrame -3 (- strand)
βHow to use the Translate DNA tool
- 1Paste your nucleotide sequence (FASTA is accepted).
- 2Pick a single reading frame or choose six-frame translation.
- 3Select one- or three-letter amino-acid output and read the protein; stop codons appear as '*'.
Frequently asked questions
- Which genetic code is used?
- The Standard Genetic Code (NCBI translation table 1). Stop codons are shown as '*'.
- What does six-frame translation mean?
- There are three reading frames on the forward strand and three on the reverse strand. Six-frame translation shows the protein for all of them so you can spot the correct ORF.
- What does 'X' mean in the output?
- X marks a codon that contains an ambiguous base (e.g. N) and therefore cannot be unambiguously translated.