About Codon Optimizer · how to use, FAQ ▾
Codon-optimise a protein or coding DNA sequence for expression in E. coli, human, yeast, CHO, Pichia and more using most-frequent-codon usage tables. A useful starting point when designing a synthetic gene or boosting recombinant protein expression — then refine with GC, repeat and secondary-structure checks before synthesis.
How to use Codon Optimizer
- 1Paste a protein sequence or a coding DNA sequence.
- 2Choose the target expression organism (E. coli, human, yeast, CHO, Pichia and more).
- 3Copy the optimised coding sequence and review its GC content.
Frequently asked questions
How does the optimisation work?
For each amino acid, the most frequently used codon in the target organism is selected. Codon usage tables are reference approximations derived from the Kazusa database.
Can I paste DNA instead of protein?
Yes. If the input looks like a nucleotide sequence it is translated (frame +1, to the first stop) before optimisation.
Should I rely on this for ordering a gene?
Treat it as a starting point. Real optimisation also considers GC content windows, secondary structure, repeats and restriction sites — verify before synthesis.