SeqBench

DNA Sequence Analyzer — Complete Sequence Report

Generate composition, ORF, restriction-site and primer summaries from one sequence.

🔒 Nothing you paste is logged or stored on our servers

Paste a DNA sequence and generate a structured first-pass analysis. The Sequence Analyzer reports length and GC content, finds open reading frames and their translated proteins, lists restriction enzymes that cut exactly once, and estimates melting temperatures for primers taken from each end. You can copy or download the complete report for documentation or follow-up analysis.

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Paste a sequence above to generate the report.

The report composes several SeqBench tools — base composition, ORF finding, a restriction-enzyme scan and end-primer Tm — into one pass.

Working on a whole construct rather than one report? Open SeqStudio — the full editor, with live feature annotation, plasmid maps, restriction and primer panels, undo/redo, multi-document tabs and GenBank / SnapGene import and export.

How to use the Sequence Analyzer tool

  1. 1Paste a DNA sequence or FASTA record.
  2. 2Optionally set the minimum ORF length to report.
  3. 3Read the composition, coding potential, cloning sites and primer section, then copy or download the report.

Frequently asked questions

What does the Sequence Analyzer report?

Length and GC content, open reading frames (with the longest ORF's protein), the restriction enzymes that cut once for cloning, and the melting temperatures of naive primers from each end — composed into one report you can copy or download.

How is this different from the individual tools?

It runs the base-composition, ORF-finder, restriction-scan and primer-Tm calculations together on one input, so you get the whole picture from a single paste instead of switching between tools.

Is my data stored?

No. Your sequence is used only to compute the report and is never logged or stored.

More

Guides

References

Primer design table

PCR primer design rules for length, GC content and Tm.

PCR cycling conditions

Reference thermal-cycling steps, temperatures, times and cycle numbers.

Related tools

Cloning Simulator

Assemble fragments and design junction primers for Gibson, Golden Gate or restriction cloning.

Batch Processor

Apply one operation to every record in a multi-FASTA and export a single CSV/TSV table.

Batch Workflow

Chain several tools into a pipeline and run it over every record in a multi-FASTA.