DNA Sequence Analyzer — Complete Sequence Report
Generate composition, ORF, restriction-site and primer summaries from one sequence.
Paste a DNA sequence and generate a structured first-pass analysis. The Sequence Analyzer reports length and GC content, finds open reading frames and their translated proteins, lists restriction enzymes that cut exactly once, and estimates melting temperatures for primers taken from each end. You can copy or download the complete report for documentation or follow-up analysis.
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Paste a sequence above to generate the report.
The report composes several SeqBench tools — base composition, ORF finding, a restriction-enzyme scan and end-primer Tm — into one pass.
Design, edit, and verify complete constructsSeqStudio combines sequence editing and annotation, plasmid maps, primer/cloning/CRISPR design, Sanger verification, and GenBank/SnapGene files in one workspace.
How to use the Sequence Analyzer tool
- 1Paste a DNA sequence or FASTA record.
- 2Optionally set the minimum ORF length to report.
- 3Read the composition, coding potential, cloning sites and primer section, then copy or download the report.
Frequently asked questions
What does the Sequence Analyzer report?
Length and GC content, open reading frames (with the longest ORF's protein), the restriction enzymes that cut once for cloning, and the melting temperatures of naive primers from each end — composed into one report you can copy or download.
How is this different from the individual tools?
It runs the base-composition, ORF-finder, restriction-scan and primer-Tm calculations together on one input, so you get the whole picture from a single paste instead of switching between tools.
How is GC content calculated?
GC content is the number of G and C bases divided by the total number of bases that resolve to one pair or the other, expressed as a percentage: GC% = (G + C + S) / (A + C + G + T/U + S + W) x 100. S and W join the counts because S is always G or C and W is always A or T; codes that span both pairs are left out of the ratio entirely.
How are ambiguous bases counted?
By what they actually determine. S means G or C and W means A or T, so S counts toward GC and W counts toward AT even though neither names a specific base. Every other IUPAC code (N, R, Y, K, M, B, D, H, V) spans both a G/C and an A/T base, so it is counted separately and excluded from both totals.