How are ORFs defined here?

An ORF starts at an ATG and runs to the next in-frame stop codon (TAA/TAG/TGA). You can set a minimum protein length and optionally require a stop codon.

What do the coordinates mean?

Start and end are 1-based positions on the original forward sequence, regardless of strand, so they map directly onto your input.

ORF Finder — Open Reading Frames

Find open reading frames in all six frames and translate them.

🔒 Runs entirely in your browser — nothing is uploaded

Scan a DNA sequence for open reading frames (ORFs) across all six reading frames, set a minimum length, and translate each ORF to protein. ORF finding is the first step in spotting potential genes, validating a synthetic construct, or confirming the coding region of a cloned insert.

DNA sequence

0 bases

Min. protein length (aa)

Require stop codon

0 ORFs found

No ORFs match the current settings.

How to use the ORF Finder tool

1Paste your DNA sequence (FASTA accepted).
2Set the minimum ORF length and whether a stop codon is required.
3Review each ORF's start/end coordinates, strand, frame and translated protein.

Frequently asked questions

How are ORFs defined here?: An ORF starts at an ATG and runs to the next in-frame stop codon (TAA/TAG/TGA). You can set a minimum protein length and optionally require a stop codon.
What do the coordinates mean?: Start and end are 1-based positions on the original forward sequence, regardless of strand, so they map directly onto your input.

Learn more

Reading Frames and Open Reading Frames (ORFs), Explained →

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