MY_CONSTRUCT
0 bp
Paste a sequence to see the map.
About ORF Finder · how to use, FAQ ▾
Scan a DNA sequence for open reading frames (ORFs) across all six reading frames, set a minimum length, and translate each ORF to protein. ORF finding is the first step in spotting potential genes, validating a synthetic construct, or confirming the coding region of a cloned insert.
How to use ORF Finder
- 1Paste your DNA sequence (FASTA accepted).
- 2Set the minimum ORF length and whether a stop codon is required.
- 3Review each ORF's start/end coordinates, strand, frame and translated protein.
Frequently asked questions
How are ORFs defined here?
An ORF starts at an ATG and runs to the next in-frame stop codon (TAA/TAG/TGA). You can set a minimum protein length and optionally require a stop codon.
What do the coordinates mean?
Start and end are 1-based positions on the original forward sequence, regardless of strand, so they map directly onto your input.