Reverse Complement Tool for DNA & RNA Sequences
Get the reverse complement, complement and reverse of a DNA/RNA sequence at once.
Paste a DNA or RNA sequence and get its reverse complement, its complement and its reverse together — all three at once, with nothing to pick between them, and DNA or RNA read off the sequence itself. This reverse complement calculator is IUPAC-aware, preserves case, and accepts FASTA, making it useful for designing reverse primers, finding a complementary DNA strand, inspecting the antisense strand, or preparing an insert for cloning.
0 bases
Paste a DNA or RNA sequence above to get its reverse complement, complement and reverse.
Design, edit, and verify complete constructsSeqStudio combines sequence editing and annotation, plasmid maps, primer/cloning/CRISPR design, Sanger verification, and GenBank/SnapGene files in one workspace.
How to use the Reverse Complement tool
- 1Paste or type your DNA/RNA sequence (plain text or FASTA) into the input box.
- 2Read the reverse complement at the top, with the plain complement and the plain reverse underneath.
- 3Copy whichever one you need for primer design, cloning or sequence review.
Frequently asked questions
What is a reverse complement?
The reverse complement is the complementary strand read 5'→3'. You complement each base (A↔T, G↔C) and then reverse the order, which gives the sequence of the opposite strand.
Does it support IUPAC ambiguity codes?
Yes. Degenerate bases such as R, Y, S, W, K, M, B, D, H, V and N are complemented correctly, and case is preserved.
Can I calculate only the complementary DNA strand?
Yes. Choose the complement operation if you want the complementary strand in the same order, or reverse complement if you want the opposite strand written 5' to 3'.
Is 'reverse compliment' the same as reverse complement?
In sequence analysis the correct term is reverse complement. People sometimes type reverse compliment, but the calculation is the same opposite-strand sequence.