About Reverse Complement · how to use, FAQ ▾
Paste a DNA or RNA sequence and calculate its reverse, complement, or full reverse complement. This reverse complement calculator is IUPAC-aware, preserves case, and accepts FASTA, making it useful for designing reverse primers, finding a complementary DNA strand, inspecting the antisense strand, or preparing an insert for cloning.
How to use Reverse Complement
- 1Paste or type your DNA/RNA sequence (plain text or FASTA) into the input box.
- 2Choose whether you want the reverse, the complement, or the full reverse complement.
- 3Read the result and copy it for primer design, cloning or sequence review.
Frequently asked questions
What is a reverse complement?
The reverse complement is the complementary strand read 5'→3'. You complement each base (A↔T, G↔C) and then reverse the order, which gives the sequence of the opposite strand.
Does it support IUPAC ambiguity codes?
Yes. Degenerate bases such as R, Y, S, W, K, M, B, D, H, V and N are complemented correctly, and case is preserved.
Can I calculate only the complementary DNA strand?
Yes. Choose the complement operation if you want the complementary strand in the same order, or reverse complement if you want the opposite strand written 5' to 3'.
Is 'reverse compliment' the same as reverse complement?
In sequence analysis the correct term is reverse complement. People sometimes type reverse compliment, but the calculation is the same opposite-strand sequence.
Is my data stored?
Your sequence is sent to the SeqBench API to compute the reverse complement and is not stored. The stateless calculation is also available via the REST API and the MCP server.