Reverse Complement Online
Get the reverse, complement or reverse complement of a DNA/RNA sequence.
🔒 Runs entirely in your browser — nothing is uploaded
Paste a DNA or RNA sequence and instantly get its reverse, complement, or full reverse complement. The tool is IUPAC-aware, preserves case, and accepts FASTA — handy for designing reverse primers, inspecting the antisense strand, or preparing an insert for cloning. Every calculation happens locally in your browser.
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Result
Result appears here
How to use the Reverse Complement tool
- 1Paste or type your DNA/RNA sequence (plain text or FASTA) into the input box.
- 2Choose whether you want the reverse, the complement, or the full reverse complement.
- 3Read the result and copy it with one click — your sequence is never uploaded.
Frequently asked questions
- What is a reverse complement?
- The reverse complement is the complementary strand read 5'→3'. You complement each base (A↔T, G↔C) and then reverse the order, which gives the sequence of the opposite strand.
- Does it support IUPAC ambiguity codes?
- Yes. Degenerate bases such as R, Y, S, W, K, M, B, D, H, V and N are complemented correctly, and case is preserved.
- Is my sequence uploaded anywhere?
- No. All computation happens in your browser with JavaScript — your sequence never leaves your computer.