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About Motif Finder · how to use, FAQ ▾

Search a DNA sequence for any motif or pattern and get every position back. The motif can use IUPAC ambiguity codes (e.g. GGNCC or WGATAR), matches may overlap, and you can allow a few mismatches and scan the reverse strand too. Useful for locating binding sites, primer landing sites, recognition sequences and short sequence signatures.

How to use Motif Finder

  1. 1Paste your DNA sequence (FASTA accepted).
  2. 2Enter the motif, using IUPAC codes for degenerate positions if needed.
  3. 3Set allowed mismatches and reverse-strand search, then read the positions found.

Frequently asked questions

Can I use ambiguity codes in the motif?

Yes. IUPAC codes expand to their base sets — for example N matches any base, R matches A or G, and Y matches C or T — so degenerate motifs like WGATAR work directly.

Does it find overlapping matches?

Yes. The search slides one base at a time, so overlapping occurrences of the motif are all reported.

What does the mismatch option do?

It allows a match to differ from the motif at up to the chosen number of positions, which is useful for finding degenerate or imperfect sites.