What input formats does the batch processor accept?

A multi-FASTA file (each record starts with a '>' header line) or plain sequences with one per line. FASTA headers become the row names; when there are no headers, records are auto-named seq_1, seq_2 and so on.

Which operations can I run over every sequence?

Reverse complement, translate (frame +1 to the first stop, with amino-acid length), GC content, length, longest open reading frame (amino-acid length across all six frames, with strand and frame), primer melting temperature (Wallace or salt-adjusted by length), and restriction-site count over the curated common enzyme set.

Can I download the results as a table?

Yes. The result is a single table with a name column plus the operation's result columns; you can preview it on the page, copy it, or download it as a CSV or TSV file to open in Excel, R or pandas.

Are my sequences uploaded?

No. Every operation runs locally in your browser over each record in turn, so no sequence is sent to a server. You can also share a link that reproduces the exact input and operation.

Batch / Multi-FASTA Processor — Run One Operation Over Every Sequence

Apply one operation to every record in a multi-FASTA and export a single CSV/TSV table.

🔒 Local processing — pasted sequences are not uploaded

Run the same analysis over a whole multi-FASTA file in one pass — the batch step that is painful to do by hand or in a chat. Paste a multi-FASTA (or one sequence per line), pick an operation — reverse complement, translate, GC content, length, longest-ORF amino-acid length, primer melting temperature, or restriction-site count — and get one tidy table with a name column plus the result columns for every record. Preview it on the page and download it as CSV or TSV for Excel, R or pandas.

Multi-FASTA input (or one sequence per line)

0 records detected

Operation

Percentage of G + C bases in each sequence.

Output format

Comma-separated table for Excel, R or pandas.

Paste a multi-FASTA file (or one sequence per line) and pick an operation to build a downloadable table. Try .

Every operation runs locally in your browser over each record in turn, so no sequence is uploaded. Translate uses the standard genetic code; longest ORF scans all six frames; restriction-site count uses the curated common enzyme set; primer Tm picks the Wallace or salt-adjusted formula by length.

How to use the Batch Processor tool

1Paste a multi-FASTA file, or one sequence per line (load the example to see the format).
2Pick an operation to run over every record, and choose CSV or TSV output.
3Read the results table on the page, then copy it or download the CSV/TSV.

Frequently asked questions

What input formats does the batch processor accept?: A multi-FASTA file (each record starts with a '>' header line) or plain sequences with one per line. FASTA headers become the row names; when there are no headers, records are auto-named seq_1, seq_2 and so on.
Which operations can I run over every sequence?: Reverse complement, translate (frame +1 to the first stop, with amino-acid length), GC content, length, longest open reading frame (amino-acid length across all six frames, with strand and frame), primer melting temperature (Wallace or salt-adjusted by length), and restriction-site count over the curated common enzyme set.
Can I download the results as a table?: Yes. The result is a single table with a name column plus the operation's result columns; you can preview it on the page, copy it, or download it as a CSV or TSV file to open in Excel, R or pandas.
Are my sequences uploaded?: No. Every operation runs locally in your browser over each record in turn, so no sequence is sent to a server. You can also share a link that reproduces the exact input and operation.

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