Insect (S. frugiperda / Sf9) Codon Usage

For every amino acid with more than one synonymous codon, Insect (S. frugiperda / Sf9)uses some codons far more often than others. The table below shows each codon's usage fraction — the share of that amino acid's codons it accounts for — with the preferred (most-used) codon highlighted.

Amino acid	1-letter	3-letter	Codon	Fraction
Alanine	A	Ala	GCT	35%preferred
Alanine	A	Ala	GCC	29%
Alanine	A	Ala	GCA	18%
Alanine	A	Ala	GCG	18%
Arginine	R	Arg	CGT	24%
Arginine	R	Arg	CGC	24%preferred
Arginine	R	Arg	CGA	8%
Arginine	R	Arg	CGG	6%
Arginine	R	Arg	AGA	19%
Arginine	R	Arg	AGG	19%
Asparagine	N	Asn	AAT	32%
Asparagine	N	Asn	AAC	68%preferred
Aspartic acid	D	Asp	GAT	40%
Aspartic acid	D	Asp	GAC	60%preferred
Cysteine	C	Cys	TGT	39%
Cysteine	C	Cys	TGC	61%preferred
Glutamic acid	E	Glu	GAA	45%
Glutamic acid	E	Glu	GAG	55%preferred
Glutamine	Q	Gln	CAA	43%
Glutamine	Q	Gln	CAG	57%preferred
Glycine	G	Gly	GGT	34%preferred
Glycine	G	Gly	GGC	31%
Glycine	G	Gly	GGA	28%
Glycine	G	Gly	GGG	7%
Histidine	H	His	CAT	36%
Histidine	H	His	CAC	64%preferred
Isoleucine	I	Ile	ATT	30%
Isoleucine	I	Ile	ATC	54%preferred
Isoleucine	I	Ile	ATA	16%
Leucine	L	Leu	TTA	10%
Leucine	L	Leu	TTG	20%
Leucine	L	Leu	CTT	12%
Leucine	L	Leu	CTC	21%
Leucine	L	Leu	CTA	9%
Leucine	L	Leu	CTG	30%preferred
Lysine	K	Lys	AAA	35%
Lysine	K	Lys	AAG	65%preferred
Methionine	M	Met	ATG	100%preferred
Phenylalanine	F	Phe	TTT	27%
Phenylalanine	F	Phe	TTC	73%preferred
Proline	P	Pro	CCT	29%preferred
Proline	P	Pro	CCC	28%
Proline	P	Pro	CCA	28%
Proline	P	Pro	CCG	16%
Serine	S	Ser	TCT	17%
Serine	S	Ser	TCC	21%preferred
Serine	S	Ser	TCA	17%
Serine	S	Ser	TCG	13%
Serine	S	Ser	AGT	14%
Serine	S	Ser	AGC	18%
Threonine	T	Thr	ACT	27%
Threonine	T	Thr	ACC	32%preferred
Threonine	T	Thr	ACA	23%
Threonine	T	Thr	ACG	18%
Tryptophan	W	Trp	TGG	100%preferred
Tyrosine	Y	Tyr	TAT	29%
Tyrosine	Y	Tyr	TAC	71%preferred
Valine	V	Val	GTT	20%
Valine	V	Val	GTC	29%
Valine	V	Val	GTA	17%
Valine	V	Val	GTG	34%preferred
Stop	*	Stop	TAA	63%
Stop	*	Stop	TAG	18%
Stop	*	Stop	TGA	18%

Optimize a protein for Insect

Paste a protein sequence and every residue is rewritten with its preferred Insect codon — the same engine behind the full Codon Optimizer tool, scoped to this one organism.

Optimize a protein for Insect

Frequently asked questions

What is the preferred codon for each amino acid in Insect?

The codon with the highest usage fraction for that amino acid in Insect (S. frugiperda / Sf9) — highlighted in green in the table below. It's the codon a codon optimizer picks when rewriting a gene for this host.

What's a rare codon to avoid in Insect?

CGG (Arginine) is used only 6% of the time among its synonymous codons in Insect (S. frugiperda / Sf9) — a run of codons like this can slow translation if the matching tRNA is scarce.

How do I codon-optimize a gene for Insect?

Paste the protein sequence into the tool below (or the full Codon Optimizer), and every residue is rewritten with its most-used codon in Insect (S. frugiperda / Sf9) — the encoded protein is unchanged, only the DNA sequence changes.

Are these values exact?

These fractions are reference approximations derived from the Kazusa Codon Usage Database for Insect (S. frugiperda / Sf9). For critical work, verify against your specific expression system rather than treating any single table as definitive.