CHO (C. griseus) Codon Usage

For every amino acid with more than one synonymous codon, CHO (C. griseus)uses some codons far more often than others. The table below shows each codon's usage fraction — the share of that amino acid's codons it accounts for — with the preferred (most-used) codon highlighted.

Amino acid	1-letter	3-letter	Codon	Fraction
Alanine	A	Ala	GCT	32%
Alanine	A	Ala	GCC	37%preferred
Alanine	A	Ala	GCA	23%
Alanine	A	Ala	GCG	7%
Arginine	R	Arg	CGT	11%
Arginine	R	Arg	CGC	18%
Arginine	R	Arg	CGA	14%
Arginine	R	Arg	CGG	19%
Arginine	R	Arg	AGA	19%
Arginine	R	Arg	AGG	19%preferred
Asparagine	N	Asn	AAT	45%
Asparagine	N	Asn	AAC	55%preferred
Aspartic acid	D	Asp	GAT	47%
Aspartic acid	D	Asp	GAC	53%preferred
Cysteine	C	Cys	TGT	47%
Cysteine	C	Cys	TGC	53%preferred
Glutamic acid	E	Glu	GAA	41%
Glutamic acid	E	Glu	GAG	59%preferred
Glutamine	Q	Gln	CAA	24%
Glutamine	Q	Gln	CAG	76%preferred
Glycine	G	Gly	GGT	20%
Glycine	G	Gly	GGC	34%preferred
Glycine	G	Gly	GGA	25%
Glycine	G	Gly	GGG	21%
Histidine	H	His	CAT	44%
Histidine	H	His	CAC	56%preferred
Isoleucine	I	Ile	ATT	35%
Isoleucine	I	Ile	ATC	51%preferred
Isoleucine	I	Ile	ATA	14%
Leucine	L	Leu	TTA	6%
Leucine	L	Leu	TTG	14%
Leucine	L	Leu	CTT	13%
Leucine	L	Leu	CTC	19%
Leucine	L	Leu	CTA	8%
Leucine	L	Leu	CTG	39%preferred
Lysine	K	Lys	AAA	39%
Lysine	K	Lys	AAG	61%preferred
Methionine	M	Met	ATG	100%preferred
Phenylalanine	F	Phe	TTT	47%
Phenylalanine	F	Phe	TTC	53%preferred
Proline	P	Pro	CCT	31%
Proline	P	Pro	CCC	32%preferred
Proline	P	Pro	CCA	29%
Proline	P	Pro	CCG	8%
Serine	S	Ser	TCT	22%
Serine	S	Ser	TCC	22%preferred
Serine	S	Ser	TCA	14%
Serine	S	Ser	TCG	5%
Serine	S	Ser	AGT	15%
Serine	S	Ser	AGC	22%
Threonine	T	Thr	ACT	26%
Threonine	T	Thr	ACC	37%preferred
Threonine	T	Thr	ACA	29%
Threonine	T	Thr	ACG	8%
Tryptophan	W	Trp	TGG	100%preferred
Tyrosine	Y	Tyr	TAT	44%
Tyrosine	Y	Tyr	TAC	56%preferred
Valine	V	Val	GTT	18%
Valine	V	Val	GTC	24%
Valine	V	Val	GTA	12%
Valine	V	Val	GTG	46%preferred
Stop	*	Stop	TAA	26%
Stop	*	Stop	TAG	24%
Stop	*	Stop	TGA	50%

Optimize a protein for CHO

Paste a protein sequence and every residue is rewritten with its preferred CHO codon — the same engine behind the full Codon Optimizer tool, scoped to this one organism.

Optimize a protein for CHO

Frequently asked questions

What is the preferred codon for each amino acid in CHO?

The codon with the highest usage fraction for that amino acid in CHO (C. griseus) — highlighted in green in the table below. It's the codon a codon optimizer picks when rewriting a gene for this host.

What's a rare codon to avoid in CHO?

TCG (Serine) is used only 5% of the time among its synonymous codons in CHO (C. griseus) — a run of codons like this can slow translation if the matching tRNA is scarce.

How do I codon-optimize a gene for CHO?

Paste the protein sequence into the tool below (or the full Codon Optimizer), and every residue is rewritten with its most-used codon in CHO (C. griseus) — the encoded protein is unchanged, only the DNA sequence changes.

Are these values exact?

These fractions are reference approximations derived from the Kazusa Codon Usage Database for CHO (C. griseus). For critical work, verify against your specific expression system rather than treating any single table as definitive.